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A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a
Various methods for editing specific sites in the Escherichia coli chromosome are available, and gene-size (∼1 kb) integration into a single site or to introduce deletions, short insertions or point mutations into multiple sites can be conducted in a short period of time. However, a method for rapid...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189296/ https://www.ncbi.nlm.nih.gov/pubmed/30356638 http://dx.doi.org/10.3389/fmicb.2018.02307 |