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Optimization of single strand DNA incorporation reaction by Moloney murine leukaemia virus reverse transcriptase

In this study, we investigated CIS reaction (clamping-mediated incorporation of single-stranded DNA with concomitant DNA syntheses) of Moloney murine leukaemia virus reverse transcriptase (MMLV-RT), and established a set of conditions with which single-stranded DNA is ligated to a G-tailed model sub...

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Detalles Bibliográficos
Autores principales: Ohtsubo, Yoshiyuki, Sasaki, Haruna, Nagata, Yuji, Tsuda, Masataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191310/
https://www.ncbi.nlm.nih.gov/pubmed/29897438
http://dx.doi.org/10.1093/dnares/dsy018