Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes

BACKGROUND: Insertion of engineered DNA fragments into bacterial vectors is the foundation of recombinant DNA technology, yet existing methods are still laborious, require many steps, depend on specific vector configuration, or require expensive reagents. RESULTS: We have developed a method, called...

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Detalles Bibliográficos
Autores principales: Fischer, Matthew D., Mgboji, Emmanuel, Liu, Zhongchi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192209/
https://www.ncbi.nlm.nih.gov/pubmed/30349582
http://dx.doi.org/10.1186/s13007-018-0359-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192209/
https://www.ncbi.nlm.nih.gov/pubmed/30349582
http://dx.doi.org/10.1186/s13007-018-0359-7

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