Cargando…
Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes
BACKGROUND: Insertion of engineered DNA fragments into bacterial vectors is the foundation of recombinant DNA technology, yet existing methods are still laborious, require many steps, depend on specific vector configuration, or require expensive reagents. RESULTS: We have developed a method, called...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2018
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192209/ https://www.ncbi.nlm.nih.gov/pubmed/30349582 http://dx.doi.org/10.1186/s13007-018-0359-7 |