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Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML

Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was al...

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Autores principales: Stamps, Blake W., Nunn, Heather S., Petryshyn, Victoria A., Oremland, Ronald S., Miller, Laurence G., Rosen, Michael R., Bauer, Kohen W., Thompson, Katharine J., Tookmanian, Elise M., Waldeck, Anna R., Loyd, Sean J., Johnson, Hope A., Stevenson, Bradley S., Berelson, William M., Corsetti, Frank A., Spear, John R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193381/
https://www.ncbi.nlm.nih.gov/pubmed/30120120
http://dx.doi.org/10.1128/AEM.01171-18
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author Stamps, Blake W.
Nunn, Heather S.
Petryshyn, Victoria A.
Oremland, Ronald S.
Miller, Laurence G.
Rosen, Michael R.
Bauer, Kohen W.
Thompson, Katharine J.
Tookmanian, Elise M.
Waldeck, Anna R.
Loyd, Sean J.
Johnson, Hope A.
Stevenson, Bradley S.
Berelson, William M.
Corsetti, Frank A.
Spear, John R.
author_facet Stamps, Blake W.
Nunn, Heather S.
Petryshyn, Victoria A.
Oremland, Ronald S.
Miller, Laurence G.
Rosen, Michael R.
Bauer, Kohen W.
Thompson, Katharine J.
Tookmanian, Elise M.
Waldeck, Anna R.
Loyd, Sean J.
Johnson, Hope A.
Stevenson, Bradley S.
Berelson, William M.
Corsetti, Frank A.
Spear, John R.
author_sort Stamps, Blake W.
collection PubMed
description Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a multiyear-long drought. These conditions presented a unique sampling opportunity to investigate microbiological dynamics and potential metabolic function during an intense natural algal bloom. We conducted a comprehensive molecular analysis along a depth transect near the center of the lake from the surface to a depth of 25 m in June 2016. Across sampled depths, rRNA gene sequencing revealed that Picocystis-associated chloroplasts were found at 40 to 50% relative abundance, greater than values recorded previously. Despite high relative abundances of the photosynthetic oxygenic algal genus Picocystis, oxygen declined below detectable limits below a depth of 15 m, corresponding with an increase in microorganisms known to be anaerobic. In contrast to previously sampled years, both metagenomic and metatranscriptomic data suggested a depletion of anaerobic sulfate-reducing microorganisms throughout the lake's water column. Transcripts associated with photosystem I and II were expressed at both 2 m and 25 m, suggesting that limited oxygen production could occur at extremely low light levels at depth within the lake. Blooms of Picocystis appear to correspond with a loss of microbial activity such as sulfate reduction within Mono Lake, yet microorganisms may survive within the sediment to repopulate the lake water column as the bloom subsides. IMPORTANCE Mono Lake, California, provides a habitat to a unique ecological community that is heavily stressed due to recent human water diversions and a period of extended drought. To date, no baseline information exists from Mono Lake to understand how the microbial community responds to human-influenced drought or algal bloom or what metabolisms are lost in the water column as a consequence of such environmental pressures. While previously identified anaerobic members of the microbial community disappear from the water column during drought and bloom, sediment samples suggest that these microorganisms survive at the lake bottom or in the subsurface. Thus, the sediments may represent a type of seed bank that could restore the microbial community as a bloom subsides. Our work sheds light on the potential photosynthetic activity of the halotolerant alga Picocystis sp. strain ML and how the function and activity of the remainder of the microbial community responds during a bloom at Mono Lake.
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spelling pubmed-61933812019-04-17 Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML Stamps, Blake W. Nunn, Heather S. Petryshyn, Victoria A. Oremland, Ronald S. Miller, Laurence G. Rosen, Michael R. Bauer, Kohen W. Thompson, Katharine J. Tookmanian, Elise M. Waldeck, Anna R. Loyd, Sean J. Johnson, Hope A. Stevenson, Bradley S. Berelson, William M. Corsetti, Frank A. Spear, John R. Appl Environ Microbiol Environmental Microbiology Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a multiyear-long drought. These conditions presented a unique sampling opportunity to investigate microbiological dynamics and potential metabolic function during an intense natural algal bloom. We conducted a comprehensive molecular analysis along a depth transect near the center of the lake from the surface to a depth of 25 m in June 2016. Across sampled depths, rRNA gene sequencing revealed that Picocystis-associated chloroplasts were found at 40 to 50% relative abundance, greater than values recorded previously. Despite high relative abundances of the photosynthetic oxygenic algal genus Picocystis, oxygen declined below detectable limits below a depth of 15 m, corresponding with an increase in microorganisms known to be anaerobic. In contrast to previously sampled years, both metagenomic and metatranscriptomic data suggested a depletion of anaerobic sulfate-reducing microorganisms throughout the lake's water column. Transcripts associated with photosystem I and II were expressed at both 2 m and 25 m, suggesting that limited oxygen production could occur at extremely low light levels at depth within the lake. Blooms of Picocystis appear to correspond with a loss of microbial activity such as sulfate reduction within Mono Lake, yet microorganisms may survive within the sediment to repopulate the lake water column as the bloom subsides. IMPORTANCE Mono Lake, California, provides a habitat to a unique ecological community that is heavily stressed due to recent human water diversions and a period of extended drought. To date, no baseline information exists from Mono Lake to understand how the microbial community responds to human-influenced drought or algal bloom or what metabolisms are lost in the water column as a consequence of such environmental pressures. While previously identified anaerobic members of the microbial community disappear from the water column during drought and bloom, sediment samples suggest that these microorganisms survive at the lake bottom or in the subsurface. Thus, the sediments may represent a type of seed bank that could restore the microbial community as a bloom subsides. Our work sheds light on the potential photosynthetic activity of the halotolerant alga Picocystis sp. strain ML and how the function and activity of the remainder of the microbial community responds during a bloom at Mono Lake. American Society for Microbiology 2018-10-17 /pmc/articles/PMC6193381/ /pubmed/30120120 http://dx.doi.org/10.1128/AEM.01171-18 Text en Copyright © 2018 Stamps et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Environmental Microbiology
Stamps, Blake W.
Nunn, Heather S.
Petryshyn, Victoria A.
Oremland, Ronald S.
Miller, Laurence G.
Rosen, Michael R.
Bauer, Kohen W.
Thompson, Katharine J.
Tookmanian, Elise M.
Waldeck, Anna R.
Loyd, Sean J.
Johnson, Hope A.
Stevenson, Bradley S.
Berelson, William M.
Corsetti, Frank A.
Spear, John R.
Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML
title Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML
title_full Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML
title_fullStr Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML
title_full_unstemmed Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML
title_short Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML
title_sort metabolic capability and phylogenetic diversity of mono lake during a bloom of the eukaryotic phototroph picocystis sp. strain ml
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193381/
https://www.ncbi.nlm.nih.gov/pubmed/30120120
http://dx.doi.org/10.1128/AEM.01171-18
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