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Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice

Real time in vivo methods are needed to better understand the interplay between diet and the gastrointestinal microbiota. Therefore, a rodent indirect calorimetry system was equipped with hydrogen (H(2)) and methane (CH(4)) sensors. H(2) production was readily detected in C57BL/6J mice and followed...

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Autores principales: Fernández-Calleja, José M. S., Konstanti, Prokopis, Swarts, Hans J. M., Bouwman, Lianne M. S., Garcia-Campayo, Vicenta, Billecke, Nils, Oosting, Annemarie, Smidt, Hauke, Keijer, Jaap, van Schothorst, Evert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193968/
https://www.ncbi.nlm.nih.gov/pubmed/30337551
http://dx.doi.org/10.1038/s41598-018-33619-0
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author Fernández-Calleja, José M. S.
Konstanti, Prokopis
Swarts, Hans J. M.
Bouwman, Lianne M. S.
Garcia-Campayo, Vicenta
Billecke, Nils
Oosting, Annemarie
Smidt, Hauke
Keijer, Jaap
van Schothorst, Evert M.
author_facet Fernández-Calleja, José M. S.
Konstanti, Prokopis
Swarts, Hans J. M.
Bouwman, Lianne M. S.
Garcia-Campayo, Vicenta
Billecke, Nils
Oosting, Annemarie
Smidt, Hauke
Keijer, Jaap
van Schothorst, Evert M.
author_sort Fernández-Calleja, José M. S.
collection PubMed
description Real time in vivo methods are needed to better understand the interplay between diet and the gastrointestinal microbiota. Therefore, a rodent indirect calorimetry system was equipped with hydrogen (H(2)) and methane (CH(4)) sensors. H(2) production was readily detected in C57BL/6J mice and followed a circadian rhythm. H(2) production was increased within 12 hours after first exposure to a lowly-digestible starch diet (LDD) compared to a highly-digestible starch diet (HDD). Marked differences were observed in the faecal microbiota of animals fed the LDD and HDD diets. H(2) was identified as a key variable explaining the variation in microbial communities, with specific taxa (including Bacteroides and Parasutterella) correlating with H(2) production upon LDD-feeding. CH(4) production was undetectable which was in line with absence of CH(4) producers in the gut. We conclude that real-time in vivo monitoring of gases provides a non-invasive time-resolved system to explore the interplay between nutrition and gut microbes in a mouse model, and demonstrates potential for translation to other animal models and human studies.
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spelling pubmed-61939682018-10-24 Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice Fernández-Calleja, José M. S. Konstanti, Prokopis Swarts, Hans J. M. Bouwman, Lianne M. S. Garcia-Campayo, Vicenta Billecke, Nils Oosting, Annemarie Smidt, Hauke Keijer, Jaap van Schothorst, Evert M. Sci Rep Article Real time in vivo methods are needed to better understand the interplay between diet and the gastrointestinal microbiota. Therefore, a rodent indirect calorimetry system was equipped with hydrogen (H(2)) and methane (CH(4)) sensors. H(2) production was readily detected in C57BL/6J mice and followed a circadian rhythm. H(2) production was increased within 12 hours after first exposure to a lowly-digestible starch diet (LDD) compared to a highly-digestible starch diet (HDD). Marked differences were observed in the faecal microbiota of animals fed the LDD and HDD diets. H(2) was identified as a key variable explaining the variation in microbial communities, with specific taxa (including Bacteroides and Parasutterella) correlating with H(2) production upon LDD-feeding. CH(4) production was undetectable which was in line with absence of CH(4) producers in the gut. We conclude that real-time in vivo monitoring of gases provides a non-invasive time-resolved system to explore the interplay between nutrition and gut microbes in a mouse model, and demonstrates potential for translation to other animal models and human studies. Nature Publishing Group UK 2018-10-18 /pmc/articles/PMC6193968/ /pubmed/30337551 http://dx.doi.org/10.1038/s41598-018-33619-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fernández-Calleja, José M. S.
Konstanti, Prokopis
Swarts, Hans J. M.
Bouwman, Lianne M. S.
Garcia-Campayo, Vicenta
Billecke, Nils
Oosting, Annemarie
Smidt, Hauke
Keijer, Jaap
van Schothorst, Evert M.
Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
title Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
title_full Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
title_fullStr Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
title_full_unstemmed Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
title_short Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
title_sort non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193968/
https://www.ncbi.nlm.nih.gov/pubmed/30337551
http://dx.doi.org/10.1038/s41598-018-33619-0
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