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Catalytic Performance of a Class III Old Yellow Enzyme and Its Cysteine Variants

Class III old yellow enzymes (OYEs) contain a conserved cysteine in their active sites. To address the role of this cysteine in OYE-mediated asymmetric synthesis, we have studied the biocatalytic properties of OYERo2a from Rhodococcus opacus 1CP (WT) as well as its engineered variants C25A, C25S and...

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Detalles Bibliográficos
Autores principales: Scholtissek, Anika, Gädke, Eric, Paul, Caroline E., Westphal, Adrie H., van Berkel, Willem J. H., Tischler, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194350/
https://www.ncbi.nlm.nih.gov/pubmed/30369915
http://dx.doi.org/10.3389/fmicb.2018.02410