A simple approach for multi-targeted shRNA-mediated inducible knockdowns using Sleeping Beauty vectors

shRNA expression is an established technique to transiently or permanently deplete cells of a particular mRNA/protein. In functional analyses of oncogenic pathways it can thus be used as an alternative to pharmacologic inhibitors, or as a means to address otherwise undruggable targets. Here we descr...

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Detalles Bibliográficos
Autores principales: Fink, Severin, Zugelder, Laurens, Roth, Bernhard, Brandt, Evelyn, Meloche, Sylvain, Izsvák, Zsuzsanna, Bargou, Ralf C., Stühmer, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195277/
https://www.ncbi.nlm.nih.gov/pubmed/30339711
http://dx.doi.org/10.1371/journal.pone.0205585

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195277/
https://www.ncbi.nlm.nih.gov/pubmed/30339711
http://dx.doi.org/10.1371/journal.pone.0205585

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