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A simple approach for multi-targeted shRNA-mediated inducible knockdowns using Sleeping Beauty vectors
shRNA expression is an established technique to transiently or permanently deplete cells of a particular mRNA/protein. In functional analyses of oncogenic pathways it can thus be used as an alternative to pharmacologic inhibitors, or as a means to address otherwise undruggable targets. Here we descr...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195277/ https://www.ncbi.nlm.nih.gov/pubmed/30339711 http://dx.doi.org/10.1371/journal.pone.0205585 |