Cargando…

High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris

BACKGROUND: The fungal ribotoxin hirsutellin A (HtA) exhibits strong insecticidal activity; however, efficient systems for expressing recombinant HtA (rHtA) are lacking. Here, we established an efficient heterologous expression system to produce large amounts of rHtA. RESULTS: Recombinant Pichia pas...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Hongbo, Xia, Yuxian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195745/ https://www.ncbi.nlm.nih.gov/pubmed/30342541 http://dx.doi.org/10.1186/s12934-018-0992-x

Ejemplares similares

Hirsutellin A: A Paradigmatic Example of the Insecticidal Function of Fungal Ribotoxins
por: Herrero-Galán, Elías, et al.
Publicado: (2013)

The transcriptome of the entomopathogenic fungus Culicinomyces clavisporus contains an ortholog of the insecticidal ribotoxin Hirsutellin
por: Foresman, Dana, et al.
Publicado: (2023)

Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUT(s) Strain
por: Rodrigues, David, et al.
Publicado: (2019)

Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation
por: Shang, Tingting, et al.
Publicado: (2017)

Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen
por: Gurramkonda, Chandrasekhar, et al.
Publicado: (2009)

A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains
por: Dietzsch, Christian, et al.
Publicado: (2011)

Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris
por: Maurer, Michael, et al.
Publicado: (2006)

HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
por: Zepeda, Andrea B., et al.
Publicado: (2014)

Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
por: Jahic, Mehmedalija, et al.
Publicado: (2003)

Optimisation of Recombinant Myrosinase Production in Pichia pastoris
por: Rosenbergová, Zuzana, et al.
Publicado: (2021)

Improved Production of Recombinant Myrosinase in Pichia pastoris
por: Rosenbergová, Zuzana, et al.
Publicado: (2021)

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures
por: Burgard, Jonas, et al.
Publicado: (2017)

Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase
por: Gmeiner, Christoph, et al.
Publicado: (2015)

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy
por: Zhang, Chengbo, et al.
Publicado: (2020)

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate
por: Jacobs, Pieter P, et al.
Publicado: (2010)

Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system
por: Ruottinen, Maria, et al.
Publicado: (2008)

Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
por: Gurramkonda, Chandrasekhar, et al.
Publicado: (2010)

The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time
por: Burgard, Jonas, et al.
Publicado: (2019)

Rapid production of pure recombinant actin isoforms in Pichia pastoris
por: Hatano, Tomoyuki, et al.
Publicado: (2018)

Production of recombinant human epidermal growth factor in Pichia pastoris
por: Eissazadeh, Samira, et al.
Publicado: (2017)

Applications of recombinant Pichia pastoris in the healthcare industry
por: Weinacker, Daniel, et al.
Publicado: (2014)

The effect of hypoxia on the lipidome of recombinant Pichia pastoris
por: Adelantado, Núria, et al.
Publicado: (2017)

Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures
por: Vanz, Ana Letícia, et al.
Publicado: (2014)

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris
por: Gurkan, Cemal, et al.
Publicado: (2005)

Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris
por: Krainer, Florian W, et al.
Publicado: (2015)

Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
por: Conacher, C. G., et al.
Publicado: (2019)

Current advances of Pichia pastoris as cell factories for production of recombinant proteins
por: Pan, Yingjie, et al.
Publicado: (2022)

Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris
por: Giesselmann, Esther, et al.
Publicado: (2017)

Effect of Codon Optimisation on the Production of Recombinant Fish Growth Hormone in Pichia pastoris
por: Rothan, Hussin A., et al.
Publicado: (2014)

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production
por: Chen, Ming-Tang, et al.
Publicado: (2012)

Upscale of recombinant α-L-rhamnosidase production by Pichia pastoris Mut(S) strain
por: Markošová, Kristína, et al.
Publicado: (2015)

Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications
por: Parashar, Deepak, et al.
Publicado: (2017)

Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris
por: Wang, Shengjun, et al.
Publicado: (2020)

Magnetic Immobilization of Pichia pastoris Cells for the Production of Recombinant Human Serum Albumin
por: Taghizadeh, Seyedeh-Masoumeh, et al.
Publicado: (2020)

Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol
por: Fina, Albert, et al.
Publicado: (2021)

Effects of Temperature and pH on Recombinant Thaumatin II Production by Pichia pastoris
por: Joseph, Jewel Ann, et al.
Publicado: (2022)

Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris
por: Roth, Gustavo, et al.
Publicado: (2018)

Developing a metabolic model‐based fed‐batch feeding strategy for Pichia pastoris fermentation through fine‐tuning of the methanol utilization pathway
por: Boojari, Mohammad Amin, et al.
Publicado: (2023)

Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale
por: Kaushik, Neha, et al.
Publicado: (2020)

Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
por: Zepeda, Andrea B., et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_m477csalgrcpumtdna50nrs18l