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An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to unders...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197777/ https://www.ncbi.nlm.nih.gov/pubmed/30364607 http://dx.doi.org/10.1016/j.mex.2018.10.009 |