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An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae

Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to unders...

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Detalles Bibliográficos
Autores principales: Bresciani, Erica, Broadbridge, Elizabeth, Liu, Paul P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197777/
https://www.ncbi.nlm.nih.gov/pubmed/30364607
http://dx.doi.org/10.1016/j.mex.2018.10.009
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author Bresciani, Erica
Broadbridge, Elizabeth
Liu, Paul P.
author_facet Bresciani, Erica
Broadbridge, Elizabeth
Liu, Paul P.
author_sort Bresciani, Erica
collection PubMed
description Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing.
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spelling pubmed-61977772018-10-25 An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae Bresciani, Erica Broadbridge, Elizabeth Liu, Paul P. MethodsX Biochemistry, Genetics and Molecular Biology Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing. Elsevier 2018-10-10 /pmc/articles/PMC6197777/ /pubmed/30364607 http://dx.doi.org/10.1016/j.mex.2018.10.009 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Bresciani, Erica
Broadbridge, Elizabeth
Liu, Paul P.
An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
title An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
title_full An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
title_fullStr An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
title_full_unstemmed An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
title_short An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
title_sort efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197777/
https://www.ncbi.nlm.nih.gov/pubmed/30364607
http://dx.doi.org/10.1016/j.mex.2018.10.009
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