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An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to unders...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197777/ https://www.ncbi.nlm.nih.gov/pubmed/30364607 http://dx.doi.org/10.1016/j.mex.2018.10.009 |
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author | Bresciani, Erica Broadbridge, Elizabeth Liu, Paul P. |
author_facet | Bresciani, Erica Broadbridge, Elizabeth Liu, Paul P. |
author_sort | Bresciani, Erica |
collection | PubMed |
description | Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing. |
format | Online Article Text |
id | pubmed-6197777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61977772018-10-25 An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae Bresciani, Erica Broadbridge, Elizabeth Liu, Paul P. MethodsX Biochemistry, Genetics and Molecular Biology Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing. Elsevier 2018-10-10 /pmc/articles/PMC6197777/ /pubmed/30364607 http://dx.doi.org/10.1016/j.mex.2018.10.009 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Bresciani, Erica Broadbridge, Elizabeth Liu, Paul P. An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae |
title | An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae |
title_full | An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae |
title_fullStr | An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae |
title_full_unstemmed | An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae |
title_short | An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae |
title_sort | efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197777/ https://www.ncbi.nlm.nih.gov/pubmed/30364607 http://dx.doi.org/10.1016/j.mex.2018.10.009 |
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