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Editing of Genomic TNFSF9 by CRISPR-Cas9 Can Be Followed by Re-Editing of Its Transcript
The CRISPR-Cas system is a well-established RNA-guided DNA editing technique widely used to modify genomic DNA sequences. I used the CRISPR-Cas9 system to change the second and third nucleotides of the triplet TCT of human TNSFSF9 in HepG2 cells to TAG to create an amber stop codon. The TCT triplet...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Korean Society for Molecular and Cellular Biology
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199566/ https://www.ncbi.nlm.nih.gov/pubmed/30352492 http://dx.doi.org/10.14348/molcells.2018.0209 |