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Set screw homogenization of murine ocular tissue, including the whole eye

PURPOSE: To compare methods for homogenizing the mouse whole eye or retina for RNA extraction. METHODS: We tested five homogenization techniques for the whole eye and the retina. Two established shearing techniques were a version of the Potter-Elvehjem homogenizer, which uses a plastic pellet pestle...

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Detalles Bibliográficos
Autores principales: Gooding, Sarah W., Chrenek, Micah A., Ferdous, Salma, Nickerson, John M., Boatright, Jeffrey H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202007/
https://www.ncbi.nlm.nih.gov/pubmed/30405307