Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling

Regulation of gene expression is primarily controlled by changes in the proteins that occupy their regulatory elements. Chromatin immunoprecipitation can confirm a protein’s occupancy at a genomic locus but requires specific, high-quality, IP-competent antibodies against nominated proteins, limiting...

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Detalles Bibliográficos
Autores principales: Myers, Samuel A, Wright, Jason, Peckner, Ryan, Kalish, Brian T, Zhang, Feng, Carr, Steven A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202184/
https://www.ncbi.nlm.nih.gov/pubmed/29735997
http://dx.doi.org/10.1038/s41592-018-0007-1
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author Myers, Samuel A
Wright, Jason
Peckner, Ryan
Kalish, Brian T
Zhang, Feng
Carr, Steven A
author_facet Myers, Samuel A
Wright, Jason
Peckner, Ryan
Kalish, Brian T
Zhang, Feng
Carr, Steven A
author_sort Myers, Samuel A
collection PubMed
description Regulation of gene expression is primarily controlled by changes in the proteins that occupy their regulatory elements. Chromatin immunoprecipitation can confirm a protein’s occupancy at a genomic locus but requires specific, high-quality, IP-competent antibodies against nominated proteins, limiting its utility. Here, we combine, genome targeting, proximity labeling, and quantitative proteomics to develop genomic locus proteomics, a method able to identify proteins associated a specific genomic locus in native cellular contexts.
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spelling pubmed-62021842018-11-07 Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling Myers, Samuel A Wright, Jason Peckner, Ryan Kalish, Brian T Zhang, Feng Carr, Steven A Nat Methods Article Regulation of gene expression is primarily controlled by changes in the proteins that occupy their regulatory elements. Chromatin immunoprecipitation can confirm a protein’s occupancy at a genomic locus but requires specific, high-quality, IP-competent antibodies against nominated proteins, limiting its utility. Here, we combine, genome targeting, proximity labeling, and quantitative proteomics to develop genomic locus proteomics, a method able to identify proteins associated a specific genomic locus in native cellular contexts. 2018-05-07 2018-06 /pmc/articles/PMC6202184/ /pubmed/29735997 http://dx.doi.org/10.1038/s41592-018-0007-1 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Myers, Samuel A
Wright, Jason
Peckner, Ryan
Kalish, Brian T
Zhang, Feng
Carr, Steven A
Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling
title Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling
title_full Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling
title_fullStr Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling
title_full_unstemmed Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling
title_short Discovery of proteins associated with a predefined genomic locus in living cells via dCAS9-APEX-mediated proximity labeling
title_sort discovery of proteins associated with a predefined genomic locus in living cells via dcas9-apex-mediated proximity labeling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202184/
https://www.ncbi.nlm.nih.gov/pubmed/29735997
http://dx.doi.org/10.1038/s41592-018-0007-1
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