Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA

The use of next-generation sequencing (NGS) has been instrumental in advancing biological research and clinical diagnostics. To fully utilize the power of NGS, complete, uniform coverage of the entire genome is required. In this study, we identified the primary sources of bias observed in sequence c...

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Detalles Bibliográficos
Autores principales: Zhang, Aihua, Li, Shaohua, Apone, Lynne, Sun, Xiaoli, Chen, Lixin, Ettwiller, Laurence M., Langhorst, Bradley W., Noren, Christopher J., Xu, Ming-Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203771/
https://www.ncbi.nlm.nih.gov/pubmed/30367148
http://dx.doi.org/10.1038/s41598-018-34079-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203771/
https://www.ncbi.nlm.nih.gov/pubmed/30367148
http://dx.doi.org/10.1038/s41598-018-34079-2

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