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High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

BACKGROUND: Bacterial filamentation occurs when rod-shaped bacteria grow without dividing. To identify genetically encoded inhibitors of division that promote filamentation, we used cell sorting flow cytometry to enrich filamentous clones from an inducible expression library, and then identified the...

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Detalles Bibliográficos
Autores principales: Mediati, Daniel G., Burke, Catherine M., Ansari, Shirin, Harry, Elizabeth J., Duggin, Iain G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206680/
https://www.ncbi.nlm.nih.gov/pubmed/30373517
http://dx.doi.org/10.1186/s12864-018-5187-7