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A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines

BACKGROUND: The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and in vivo growth monitoring. Confocal microscopy coupled with the use of propidium...

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Detalles Bibliográficos
Autores principales: Bureau, Charlotte, Lanau, Nadège, Ingouff, Mathieu, Hassan, Boukhaddaoui, Meunier, Anne-Cécile, Divol, Fanchon, Sevilla, Rosie, Mieulet, Delphine, Dievart, Anne, Périn, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206838/
https://www.ncbi.nlm.nih.gov/pubmed/30386414
http://dx.doi.org/10.1186/s13007-018-0364-x