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Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam

Pork is the most commonly consumed meat in Vietnam, and Salmonella enterica is a common contaminant. This study aimed to assess potential S. enterica cross-contamination between raw and cooked pork in Vietnamese households. Different scenarios for cross-contamination were constructed based on a hous...

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Autores principales: Dang-Xuan, Sinh, Nguyen-Viet, Hung, Pham-Duc, Phuc, Grace, Delia, Unger, Fred, Nguyen-Hai, Nam, Nguyen-Tien, Thanh, Makita, Kohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210979/
https://www.ncbi.nlm.nih.gov/pubmed/30360454
http://dx.doi.org/10.3390/ijerph15102324
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author Dang-Xuan, Sinh
Nguyen-Viet, Hung
Pham-Duc, Phuc
Grace, Delia
Unger, Fred
Nguyen-Hai, Nam
Nguyen-Tien, Thanh
Makita, Kohei
author_facet Dang-Xuan, Sinh
Nguyen-Viet, Hung
Pham-Duc, Phuc
Grace, Delia
Unger, Fred
Nguyen-Hai, Nam
Nguyen-Tien, Thanh
Makita, Kohei
author_sort Dang-Xuan, Sinh
collection PubMed
description Pork is the most commonly consumed meat in Vietnam, and Salmonella enterica is a common contaminant. This study aimed to assess potential S. enterica cross-contamination between raw and cooked pork in Vietnamese households. Different scenarios for cross-contamination were constructed based on a household survey of pork handling practices (416 households). Overall, 71% of people used the same knife and cutting board for both raw and cooked pork; however, all washed their hands and utensils between handling raw and cooked pork. The different scenarios were experimentally tested. First, S. enterica was inoculated on raw pork and surfaces (hands, knives and cutting boards); next, water used for washing and pork were sampled to identify the presence and concentration of S. enterica during different scenarios of food preparation. Bootstrapping techniques were applied to simulate transfer rates of S. enterica cross-contamination. No cross-contamination to cooked pork was observed in the scenario of using the same hands with new cutting boards and knives. The probability of re-contamination in the scenarios involving re-using the cutting board after washing was significantly higher compared to the scenarios which used a new cutting board. Stochastic simulation found a high risk of cross-contamination from raw to cooked pork when the same hands, knives and cutting boards were used for handling raw and cooked pork (78%); when the same cutting board but a different knife was used, cross-contamination was still high (67%). Cross-contamination between was not seen when different cutting boards and knives were used for cutting raw and cooked pork. This study provided an insight into cross-contamination of S. enterica, given common food handling practices in Vietnamese households and can be used for risk assessment of pork consumption.
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spelling pubmed-62109792018-11-02 Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam Dang-Xuan, Sinh Nguyen-Viet, Hung Pham-Duc, Phuc Grace, Delia Unger, Fred Nguyen-Hai, Nam Nguyen-Tien, Thanh Makita, Kohei Int J Environ Res Public Health Article Pork is the most commonly consumed meat in Vietnam, and Salmonella enterica is a common contaminant. This study aimed to assess potential S. enterica cross-contamination between raw and cooked pork in Vietnamese households. Different scenarios for cross-contamination were constructed based on a household survey of pork handling practices (416 households). Overall, 71% of people used the same knife and cutting board for both raw and cooked pork; however, all washed their hands and utensils between handling raw and cooked pork. The different scenarios were experimentally tested. First, S. enterica was inoculated on raw pork and surfaces (hands, knives and cutting boards); next, water used for washing and pork were sampled to identify the presence and concentration of S. enterica during different scenarios of food preparation. Bootstrapping techniques were applied to simulate transfer rates of S. enterica cross-contamination. No cross-contamination to cooked pork was observed in the scenario of using the same hands with new cutting boards and knives. The probability of re-contamination in the scenarios involving re-using the cutting board after washing was significantly higher compared to the scenarios which used a new cutting board. Stochastic simulation found a high risk of cross-contamination from raw to cooked pork when the same hands, knives and cutting boards were used for handling raw and cooked pork (78%); when the same cutting board but a different knife was used, cross-contamination was still high (67%). Cross-contamination between was not seen when different cutting boards and knives were used for cutting raw and cooked pork. This study provided an insight into cross-contamination of S. enterica, given common food handling practices in Vietnamese households and can be used for risk assessment of pork consumption. MDPI 2018-10-22 2018-10 /pmc/articles/PMC6210979/ /pubmed/30360454 http://dx.doi.org/10.3390/ijerph15102324 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dang-Xuan, Sinh
Nguyen-Viet, Hung
Pham-Duc, Phuc
Grace, Delia
Unger, Fred
Nguyen-Hai, Nam
Nguyen-Tien, Thanh
Makita, Kohei
Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam
title Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam
title_full Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam
title_fullStr Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam
title_full_unstemmed Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam
title_short Simulating Cross-Contamination of Cooked Pork with Salmonella enterica from Raw Pork through Home Kitchen Preparation in Vietnam
title_sort simulating cross-contamination of cooked pork with salmonella enterica from raw pork through home kitchen preparation in vietnam
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210979/
https://www.ncbi.nlm.nih.gov/pubmed/30360454
http://dx.doi.org/10.3390/ijerph15102324
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