Primer design and amplification efficiencies are crucial for reliability of quantitative PCR studies of caffeine biosynthetic N-methyltransferases in coffee

Primers having suboptimal amplification efficiencies were shown to falsely represent fold change expression of the N-methyltransferases gene family involved in caffeine biosynthesis in Coffea canephora. To study this phenomenon, the role of stability of the internal reference gene, as well as the am...

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Detalles Bibliográficos
Autores principales: Sreedharan, Simmi P., Kumar, Avinash, Giridhar, Parvatam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2018
Materias:
Short Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212373/
https://www.ncbi.nlm.nih.gov/pubmed/30402369
http://dx.doi.org/10.1007/s13205-018-1487-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212373/
https://www.ncbi.nlm.nih.gov/pubmed/30402369
http://dx.doi.org/10.1007/s13205-018-1487-5

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