MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme

Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction. However, large DNA fragments can only be assembled by alternating use of two or more type IIS restrict...

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Detalles Bibliográficos
Autores principales: Lin, Da, O’Callaghan, Christopher A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212791/
https://www.ncbi.nlm.nih.gov/pubmed/29986052
http://dx.doi.org/10.1093/nar/gky596

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212791/
https://www.ncbi.nlm.nih.gov/pubmed/29986052
http://dx.doi.org/10.1093/nar/gky596

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