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MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme

Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction. However, large DNA fragments can only be assembled by alternating use of two or more type IIS restrict...

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Detalles Bibliográficos
Autores principales:	Lin, Da, O’Callaghan, Christopher A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212791/ https://www.ncbi.nlm.nih.gov/pubmed/29986052 http://dx.doi.org/10.1093/nar/gky596

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