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Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide

Pulmonary fibrosis is a fatal respiratory disease that gradually leads to dyspnea, mainly accompanied by excessive collagen production in the fibroblast and myofibroblast through mechanisms such as abnormal alveolar epithelial cells remodeling and stimulation of the extracellular matrix (ECM). Our r...

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Autores principales: Chuang, Hong-Meng, Ho, Li-Ing, Huang, Mao-Hsuan, Huang, Kun-Lun, Chiou, Tzyy-Wen, Lin, Shinn-Zong, Su, Hong-Lin, Harn, Horng-Jyh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213013/
https://www.ncbi.nlm.nih.gov/pubmed/30287739
http://dx.doi.org/10.3390/ijms19103024
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author Chuang, Hong-Meng
Ho, Li-Ing
Huang, Mao-Hsuan
Huang, Kun-Lun
Chiou, Tzyy-Wen
Lin, Shinn-Zong
Su, Hong-Lin
Harn, Horng-Jyh
author_facet Chuang, Hong-Meng
Ho, Li-Ing
Huang, Mao-Hsuan
Huang, Kun-Lun
Chiou, Tzyy-Wen
Lin, Shinn-Zong
Su, Hong-Lin
Harn, Horng-Jyh
author_sort Chuang, Hong-Meng
collection PubMed
description Pulmonary fibrosis is a fatal respiratory disease that gradually leads to dyspnea, mainly accompanied by excessive collagen production in the fibroblast and myofibroblast through mechanisms such as abnormal alveolar epithelial cells remodeling and stimulation of the extracellular matrix (ECM). Our results show that a small molecule, butylidenephthalide (BP), reduces type I collagen (COL1) expression in Transforming Growth Factor beta (TGF-β)-induced lung fibroblast without altering downstream pathways of TGF-β, such as Smad phosphorylation. Treatment of BP also reduces the expression of transcription factor Sex Determining Region Y-box 2 (SOX2), and the ectopic expression of SOX2 overcomes the inhibitory actions of BP on COL1 expression. We also found that serial deletion of the SOX2 binding site on 3′COL1 promoter results in a marked reduction in luciferase activity. Moreover, chromatin immunoprecipitation, which was found on the SOX2 binding site of the COL1 promoter, decreases in BP-treated cells. In an in vivo study using a bleomycin-induced pulmonary fibrosis C57BL/6 mice model, mice treated with BP displayed reduced lung fibrosis and collagen deposition, recovering in their pulmonary ventilation function. The reduction of SOX2 expression in BP-treated lung tissues is consistent with our findings in the fibroblast. This is the first report that reveals a non-canonical regulation of COL1 promoter via SOX2 binding, and contributes to the amelioration of pulmonary fibrosis by BP treatment.
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spelling pubmed-62130132018-11-14 Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide Chuang, Hong-Meng Ho, Li-Ing Huang, Mao-Hsuan Huang, Kun-Lun Chiou, Tzyy-Wen Lin, Shinn-Zong Su, Hong-Lin Harn, Horng-Jyh Int J Mol Sci Article Pulmonary fibrosis is a fatal respiratory disease that gradually leads to dyspnea, mainly accompanied by excessive collagen production in the fibroblast and myofibroblast through mechanisms such as abnormal alveolar epithelial cells remodeling and stimulation of the extracellular matrix (ECM). Our results show that a small molecule, butylidenephthalide (BP), reduces type I collagen (COL1) expression in Transforming Growth Factor beta (TGF-β)-induced lung fibroblast without altering downstream pathways of TGF-β, such as Smad phosphorylation. Treatment of BP also reduces the expression of transcription factor Sex Determining Region Y-box 2 (SOX2), and the ectopic expression of SOX2 overcomes the inhibitory actions of BP on COL1 expression. We also found that serial deletion of the SOX2 binding site on 3′COL1 promoter results in a marked reduction in luciferase activity. Moreover, chromatin immunoprecipitation, which was found on the SOX2 binding site of the COL1 promoter, decreases in BP-treated cells. In an in vivo study using a bleomycin-induced pulmonary fibrosis C57BL/6 mice model, mice treated with BP displayed reduced lung fibrosis and collagen deposition, recovering in their pulmonary ventilation function. The reduction of SOX2 expression in BP-treated lung tissues is consistent with our findings in the fibroblast. This is the first report that reveals a non-canonical regulation of COL1 promoter via SOX2 binding, and contributes to the amelioration of pulmonary fibrosis by BP treatment. MDPI 2018-10-04 /pmc/articles/PMC6213013/ /pubmed/30287739 http://dx.doi.org/10.3390/ijms19103024 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chuang, Hong-Meng
Ho, Li-Ing
Huang, Mao-Hsuan
Huang, Kun-Lun
Chiou, Tzyy-Wen
Lin, Shinn-Zong
Su, Hong-Lin
Harn, Horng-Jyh
Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide
title Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide
title_full Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide
title_fullStr Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide
title_full_unstemmed Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide
title_short Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide
title_sort non-canonical regulation of type i collagen through promoter binding of sox2 and its contribution to ameliorating pulmonary fibrosis by butylidenephthalide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213013/
https://www.ncbi.nlm.nih.gov/pubmed/30287739
http://dx.doi.org/10.3390/ijms19103024
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