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Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons
Analyzing rare DNA and RNA molecules in limited sample sizes, such as liquid biopsies and single cells, often requires preamplification, which makes downstream analyses particularly sensitive to polymerase chain reaction (PCR) generated contamination. Herein, we assessed the feasibility of performin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214100/ https://www.ncbi.nlm.nih.gov/pubmed/30332749 http://dx.doi.org/10.3390/ijms19103185 |