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Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons

Analyzing rare DNA and RNA molecules in limited sample sizes, such as liquid biopsies and single cells, often requires preamplification, which makes downstream analyses particularly sensitive to polymerase chain reaction (PCR) generated contamination. Herein, we assessed the feasibility of performin...

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Detalles Bibliográficos
Autores principales: Andersson, Daniel, Svec, David, Pedersen, Cathrine, Henriksen, Jørn Remi, Ståhlberg, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214100/
https://www.ncbi.nlm.nih.gov/pubmed/30332749
http://dx.doi.org/10.3390/ijms19103185