Cargando…

Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues

We present the results of polarimetric analysis of collagen on varying pathologies of breast tissues using second-harmonic patterned polarization-analyzed reflection confocal (SPPARC) microscopy. Experiments are conducted on a breast tissue microarray having benign tissues (BT), malignant invasive l...

Descripción completa

Detalles Bibliográficos
Autores principales: Okoro, Chukwuemeka, Kelkar, Varun, Sivaguru, Mayandi, Emmadi, Rajyasree, Toussaint, Kimani C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214917/
https://www.ncbi.nlm.nih.gov/pubmed/30389994
http://dx.doi.org/10.1038/s41598-018-34693-0
_version_ 1783368035548004352
author Okoro, Chukwuemeka
Kelkar, Varun
Sivaguru, Mayandi
Emmadi, Rajyasree
Toussaint, Kimani C.
author_facet Okoro, Chukwuemeka
Kelkar, Varun
Sivaguru, Mayandi
Emmadi, Rajyasree
Toussaint, Kimani C.
author_sort Okoro, Chukwuemeka
collection PubMed
description We present the results of polarimetric analysis of collagen on varying pathologies of breast tissues using second-harmonic patterned polarization-analyzed reflection confocal (SPPARC) microscopy. Experiments are conducted on a breast tissue microarray having benign tissues (BT), malignant invasive lobular carcinoma (ILC), and benign stroma adjacent to the malignant tissues (called the benign adjacent tissue, or BAT). Stroma in BAT and ILC exhibit the largest parameter differences. We observe that stromal collagen readings in ILC show lower depolarization, lower diattenuation and higher linear degree-of-polarization values than stromal collagen in BAT. This suggests that the optical properties of collagen change most in the vicinity of tumors. A similar trend is also exhibited in the non-collagenous extrafibrillar matrix plus cells (EFMC) region. The three highlighted parameters show greatest sensitivity to changes in the polarization response of collagen between pathologies.
format Online
Article
Text
id pubmed-6214917
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-62149172018-11-06 Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues Okoro, Chukwuemeka Kelkar, Varun Sivaguru, Mayandi Emmadi, Rajyasree Toussaint, Kimani C. Sci Rep Article We present the results of polarimetric analysis of collagen on varying pathologies of breast tissues using second-harmonic patterned polarization-analyzed reflection confocal (SPPARC) microscopy. Experiments are conducted on a breast tissue microarray having benign tissues (BT), malignant invasive lobular carcinoma (ILC), and benign stroma adjacent to the malignant tissues (called the benign adjacent tissue, or BAT). Stroma in BAT and ILC exhibit the largest parameter differences. We observe that stromal collagen readings in ILC show lower depolarization, lower diattenuation and higher linear degree-of-polarization values than stromal collagen in BAT. This suggests that the optical properties of collagen change most in the vicinity of tumors. A similar trend is also exhibited in the non-collagenous extrafibrillar matrix plus cells (EFMC) region. The three highlighted parameters show greatest sensitivity to changes in the polarization response of collagen between pathologies. Nature Publishing Group UK 2018-11-02 /pmc/articles/PMC6214917/ /pubmed/30389994 http://dx.doi.org/10.1038/s41598-018-34693-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Okoro, Chukwuemeka
Kelkar, Varun
Sivaguru, Mayandi
Emmadi, Rajyasree
Toussaint, Kimani C.
Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues
title Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues
title_full Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues
title_fullStr Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues
title_full_unstemmed Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues
title_short Second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues
title_sort second-harmonic patterned polarization-analyzed reflection confocal microscopy of stromal collagen in benign and malignant breast tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214917/
https://www.ncbi.nlm.nih.gov/pubmed/30389994
http://dx.doi.org/10.1038/s41598-018-34693-0
work_keys_str_mv AT okorochukwuemeka secondharmonicpatternedpolarizationanalyzedreflectionconfocalmicroscopyofstromalcollageninbenignandmalignantbreasttissues
AT kelkarvarun secondharmonicpatternedpolarizationanalyzedreflectionconfocalmicroscopyofstromalcollageninbenignandmalignantbreasttissues
AT sivagurumayandi secondharmonicpatternedpolarizationanalyzedreflectionconfocalmicroscopyofstromalcollageninbenignandmalignantbreasttissues
AT emmadirajyasree secondharmonicpatternedpolarizationanalyzedreflectionconfocalmicroscopyofstromalcollageninbenignandmalignantbreasttissues
AT toussaintkimanic secondharmonicpatternedpolarizationanalyzedreflectionconfocalmicroscopyofstromalcollageninbenignandmalignantbreasttissues