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Fast Optical Sectioning for Widefield Fluorescence Mesoscopy with the Mesolens based on HiLo Microscopy

We present here a fast optical sectioning method for mesoscopy based on HiLo microscopy, which makes possible imaging of specimens of up to 4.4 mm × 3 mm × 3 mm in volume in under 17 hours (estimated for a z-stack comprising 1000 images excluding computation time) with subcellular resolution through...

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Detalles Bibliográficos
Autores principales: Schniete, Jan, Franssen, Aimee, Dempster, John, Bushell, Trevor J, Amos, William Bradshaw, McConnell, Gail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215018/
https://www.ncbi.nlm.nih.gov/pubmed/30390029
http://dx.doi.org/10.1038/s41598-018-34516-2