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Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine

[Image: see text] The aggregates of α-synuclein bear a close connection with Parkinson’s disease, which is largely characterized by the loss of the dopaminergic neurons. Dopamine promotes the formation of undesirable sodium dodecyl sulfate (SDS)-resistant oligomers of α-synuclein. In this study, we...

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Autores principales: Saha, Shivnetra, Khan, Mohammad Ashhar Iqbal, Mudhara, Deepak, Deep, Shashank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217689/
https://www.ncbi.nlm.nih.gov/pubmed/30411062
http://dx.doi.org/10.1021/acsomega.8b00993
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author Saha, Shivnetra
Khan, Mohammad Ashhar Iqbal
Mudhara, Deepak
Deep, Shashank
author_facet Saha, Shivnetra
Khan, Mohammad Ashhar Iqbal
Mudhara, Deepak
Deep, Shashank
author_sort Saha, Shivnetra
collection PubMed
description [Image: see text] The aggregates of α-synuclein bear a close connection with Parkinson’s disease, which is largely characterized by the loss of the dopaminergic neurons. Dopamine promotes the formation of undesirable sodium dodecyl sulfate (SDS)-resistant oligomers of α-synuclein. In this study, we have shown that the inhibition of fibrillation by an additive may not always be the ultimate deciding factor in the context of its potential as a successful additive. Copper promotes the fibrillation of α-synuclein in buffer alone but inhibits the formation of SDS-resistant oligomers in the presence of dopamine. Glycerol, on the other hand, increases the population of such dopamine-mediated SDS-resistant oligomers. We speculate such an effect to be a manifestation of the distinct oxidation pathway of dopamine in the presence of copper.
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spelling pubmed-62176892018-11-06 Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine Saha, Shivnetra Khan, Mohammad Ashhar Iqbal Mudhara, Deepak Deep, Shashank ACS Omega [Image: see text] The aggregates of α-synuclein bear a close connection with Parkinson’s disease, which is largely characterized by the loss of the dopaminergic neurons. Dopamine promotes the formation of undesirable sodium dodecyl sulfate (SDS)-resistant oligomers of α-synuclein. In this study, we have shown that the inhibition of fibrillation by an additive may not always be the ultimate deciding factor in the context of its potential as a successful additive. Copper promotes the fibrillation of α-synuclein in buffer alone but inhibits the formation of SDS-resistant oligomers in the presence of dopamine. Glycerol, on the other hand, increases the population of such dopamine-mediated SDS-resistant oligomers. We speculate such an effect to be a manifestation of the distinct oxidation pathway of dopamine in the presence of copper. American Chemical Society 2018-10-26 /pmc/articles/PMC6217689/ /pubmed/30411062 http://dx.doi.org/10.1021/acsomega.8b00993 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Saha, Shivnetra
Khan, Mohammad Ashhar Iqbal
Mudhara, Deepak
Deep, Shashank
Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine
title Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine
title_full Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine
title_fullStr Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine
title_full_unstemmed Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine
title_short Tuning the Balance between Fibrillation and Oligomerization of α-Synuclein in the Presence of Dopamine
title_sort tuning the balance between fibrillation and oligomerization of α-synuclein in the presence of dopamine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217689/
https://www.ncbi.nlm.nih.gov/pubmed/30411062
http://dx.doi.org/10.1021/acsomega.8b00993
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