A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains

Hexose transporter-deficient yeast strains are valuable testbeds for the study of sugar transport by native and heterologous transporters. In the popular Saccharomyces cerevisiae strain EBY.VW4000, deletion of 21 transporters completely abolished hexose transport. However, repeated use of the LoxP/C...

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Detalles Bibliográficos
Autores principales: Wijsman, Melanie, Świat, Michał A, Marques, Wesley L, Hettinga, Johanna K, van den Broek, Marcel, de la Torre Cortés, Pilar, Mans, Robert, Pronk, Jack T, Daran, Jean-Marc, Daran-Lapujade, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217715/
https://www.ncbi.nlm.nih.gov/pubmed/30285096
http://dx.doi.org/10.1093/femsyr/foy107

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217715/
https://www.ncbi.nlm.nih.gov/pubmed/30285096
http://dx.doi.org/10.1093/femsyr/foy107

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