Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling

Amplification and sequencing of 16S amplicons are widely used for profiling the structure of oral microbiota. However, it remains not clear whether and to what degree DNA extraction and targeted 16S rRNA hypervariable regions influence the analysis. Based on a mock community consisting of five oral...

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Detalles Bibliográficos
Autores principales: Teng, Fei, Darveekaran Nair, Sree Sankar, Zhu, Pengfei, Li, Shanshan, Huang, Shi, Li, Xiaolan, Xu, Jian, Yang, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218491/
https://www.ncbi.nlm.nih.gov/pubmed/30397210
http://dx.doi.org/10.1038/s41598-018-34294-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218491/
https://www.ncbi.nlm.nih.gov/pubmed/30397210
http://dx.doi.org/10.1038/s41598-018-34294-x

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