Conversion of an amide to a high-energy thioester by Staphylococcus aureus sortase A is powered by variable binding affinity for calcium

Thioesters are key intermediates in biology, which often are generated from less energy-rich amide precursors. Staphylococcus aureus sortase A (SrtA) is an enzyme widely used in biotechnology for peptide ligation. The reaction proceeds in two steps, where the first step involves the conversion of an...

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Detalles Bibliográficos
Autores principales: Wang, Xiao, Chen, Jia-Liang, Otting, Gottfried, Su, Xun-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219580/
https://www.ncbi.nlm.nih.gov/pubmed/30401805
http://dx.doi.org/10.1038/s41598-018-34752-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219580/
https://www.ncbi.nlm.nih.gov/pubmed/30401805
http://dx.doi.org/10.1038/s41598-018-34752-6

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