A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues

Targeting gene function with spatial or temporal specificity is a key goal in molecular genetics. CRISPR-Cas9 has greatly facilitated this strategy, but some standard approaches are problematic. For instance, simple tissue-specific or global overexpression of Cas9 can cause significant lethality or...

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Detalles Bibliográficos
Autores principales: Huynh, Nhan, Zeng, Jie, Liu, Wen, King-Jones, Kirst
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2018
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222582/
https://www.ncbi.nlm.nih.gov/pubmed/30213867
http://dx.doi.org/10.1534/g3.118.200539

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222582/
https://www.ncbi.nlm.nih.gov/pubmed/30213867
http://dx.doi.org/10.1534/g3.118.200539

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