Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
Hepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and envi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Instituto de Medicina Tropical
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223251/ https://www.ncbi.nlm.nih.gov/pubmed/30427404 http://dx.doi.org/10.1590/S1678-9946201860069 |
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author | de Souza, Fernanda Gil da Silva, Francini Pereira Staggemeier, Rodrigo Rigotto, Caroline Spilki, Fernando Rosado |
author_facet | de Souza, Fernanda Gil da Silva, Francini Pereira Staggemeier, Rodrigo Rigotto, Caroline Spilki, Fernando Rosado |
author_sort | de Souza, Fernanda Gil |
collection | PubMed |
description | Hepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and environmental quality. The main goals in the present study were to standardize a cell culture combined to a polymerase chain reaction protocol for the detection and quantification of viral viability and analyze whether the virus could be found in water samples collected in four urban streams of Sinos River watershed. Virus recovery was assayed from known virus concentrations measured in experimentally contaminated raw and ultrapure water (MilliQ(®)). Recovery rates ranged from 270% in raw water to 15,000% in ultrapure water. In a second step, a qPCR coupled to a previous passage in cells, demonstrated more analytical sensitivity when compared to samples assayed without a previous passage in cell cultures. HAV genome was detected in only 1 of 84 samples analyzed, pointing to a very low occurrence of HAV in water samples in the studied region. These findings are remarkable, since no more than 5% of the domestic sewage in this area is treated pointing to a low occurrence of HAV in the population living nearby during the study period. |
format | Online Article Text |
id | pubmed-6223251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-62232512018-11-09 Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil de Souza, Fernanda Gil da Silva, Francini Pereira Staggemeier, Rodrigo Rigotto, Caroline Spilki, Fernando Rosado Rev Inst Med Trop Sao Paulo Original Article Hepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and environmental quality. The main goals in the present study were to standardize a cell culture combined to a polymerase chain reaction protocol for the detection and quantification of viral viability and analyze whether the virus could be found in water samples collected in four urban streams of Sinos River watershed. Virus recovery was assayed from known virus concentrations measured in experimentally contaminated raw and ultrapure water (MilliQ(®)). Recovery rates ranged from 270% in raw water to 15,000% in ultrapure water. In a second step, a qPCR coupled to a previous passage in cells, demonstrated more analytical sensitivity when compared to samples assayed without a previous passage in cell cultures. HAV genome was detected in only 1 of 84 samples analyzed, pointing to a very low occurrence of HAV in water samples in the studied region. These findings are remarkable, since no more than 5% of the domestic sewage in this area is treated pointing to a low occurrence of HAV in the population living nearby during the study period. Instituto de Medicina Tropical 2018-11-08 /pmc/articles/PMC6223251/ /pubmed/30427404 http://dx.doi.org/10.1590/S1678-9946201860069 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article de Souza, Fernanda Gil da Silva, Francini Pereira Staggemeier, Rodrigo Rigotto, Caroline Spilki, Fernando Rosado Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil |
title | Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil |
title_full | Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil |
title_fullStr | Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil |
title_full_unstemmed | Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil |
title_short | Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil |
title_sort | low occurrence of hepatitis a virus in water samples from an urban area of southern brazil |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223251/ https://www.ncbi.nlm.nih.gov/pubmed/30427404 http://dx.doi.org/10.1590/S1678-9946201860069 |
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