Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model

OBJECTIVES: To investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish. RESULTS: Here we have constructed a transgenic zebrafish strain that can be screened by pigment deficiency. Compared with the traditional CRISPR injection method, the transgenic zebrafish c...

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Autores principales: Yang, Zhe, Chen, Shihao, Xue, Songlei, Li, Xinxiu, Sun, Zhen, Yang, Yu, Hu, Xuming, Geng, Tuoyu, Cui, Hengmi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2018
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223727/
https://www.ncbi.nlm.nih.gov/pubmed/30244429
http://dx.doi.org/10.1007/s10529-018-2605-5
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author Yang, Zhe
Chen, Shihao
Xue, Songlei
Li, Xinxiu
Sun, Zhen
Yang, Yu
Hu, Xuming
Geng, Tuoyu
Cui, Hengmi
author_facet Yang, Zhe
Chen, Shihao
Xue, Songlei
Li, Xinxiu
Sun, Zhen
Yang, Yu
Hu, Xuming
Geng, Tuoyu
Cui, Hengmi
author_sort Yang, Zhe
collection PubMed
description OBJECTIVES: To investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish. RESULTS: Here we have constructed a transgenic zebrafish strain that can be screened by pigment deficiency. Compared with the traditional CRISPR injection method, the transgenic zebrafish can improve the efficiency of genome editing significantly. At the same time, we first observed that the phenotype of vertebral malformation in early embryonic development of zebrafish after ZFERV knockout. CONCLUSIONS: The transgenic zebrafish with expressed Cas9, is more efficient in genome editing. And the results of ZFERV knockout indicated that ERV may affect the vertebral development by Notch1/Delta D signal pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10529-018-2605-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-62237272018-11-18 Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model Yang, Zhe Chen, Shihao Xue, Songlei Li, Xinxiu Sun, Zhen Yang, Yu Hu, Xuming Geng, Tuoyu Cui, Hengmi Biotechnol Lett Original Research Paper OBJECTIVES: To investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish. RESULTS: Here we have constructed a transgenic zebrafish strain that can be screened by pigment deficiency. Compared with the traditional CRISPR injection method, the transgenic zebrafish can improve the efficiency of genome editing significantly. At the same time, we first observed that the phenotype of vertebral malformation in early embryonic development of zebrafish after ZFERV knockout. CONCLUSIONS: The transgenic zebrafish with expressed Cas9, is more efficient in genome editing. And the results of ZFERV knockout indicated that ERV may affect the vertebral development by Notch1/Delta D signal pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10529-018-2605-5) contains supplementary material, which is available to authorized users. Springer Netherlands 2018-09-22 2018 /pmc/articles/PMC6223727/ /pubmed/30244429 http://dx.doi.org/10.1007/s10529-018-2605-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Research Paper
Yang, Zhe
Chen, Shihao
Xue, Songlei
Li, Xinxiu
Sun, Zhen
Yang, Yu
Hu, Xuming
Geng, Tuoyu
Cui, Hengmi
Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model
title Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model
title_full Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model
title_fullStr Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model
title_full_unstemmed Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model
title_short Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model
title_sort generation of cas9 transgenic zebrafish and their application in establishing an erv-deficient animal model
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223727/
https://www.ncbi.nlm.nih.gov/pubmed/30244429
http://dx.doi.org/10.1007/s10529-018-2605-5
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