Intron retention is a source of neoepitopes in cancer

We present an in silico approach to identify neoepitopes derived from intron retention events in tumor transcriptomes. Using mass spectrometry immunopeptidome analysis, we show that retained intron (RI) neoepitopes are processed and presented on MHC-I on the surface of cancer cell lines. RNA-derived...

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Detalles Bibliográficos
Autores principales: Smart, Alicia C., Margolis, Claire A., Pimentel, Harold, He, Meng Xiao, Miao, Diana, Adeegbe, Dennis, Fugmann, Tim, Wong, Kwok-Kin, Van Allen, Eliezer M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226333/
https://www.ncbi.nlm.nih.gov/pubmed/30114007
http://dx.doi.org/10.1038/nbt.4239
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author Smart, Alicia C.
Margolis, Claire A.
Pimentel, Harold
He, Meng Xiao
Miao, Diana
Adeegbe, Dennis
Fugmann, Tim
Wong, Kwok-Kin
Van Allen, Eliezer M.
author_facet Smart, Alicia C.
Margolis, Claire A.
Pimentel, Harold
He, Meng Xiao
Miao, Diana
Adeegbe, Dennis
Fugmann, Tim
Wong, Kwok-Kin
Van Allen, Eliezer M.
author_sort Smart, Alicia C.
collection PubMed
description We present an in silico approach to identify neoepitopes derived from intron retention events in tumor transcriptomes. Using mass spectrometry immunopeptidome analysis, we show that retained intron (RI) neoepitopes are processed and presented on MHC-I on the surface of cancer cell lines. RNA-derived neoepitopes should be considered for prospective personalized cancer vaccine development.
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spelling pubmed-62263332019-02-16 Intron retention is a source of neoepitopes in cancer Smart, Alicia C. Margolis, Claire A. Pimentel, Harold He, Meng Xiao Miao, Diana Adeegbe, Dennis Fugmann, Tim Wong, Kwok-Kin Van Allen, Eliezer M. Nat Biotechnol Article We present an in silico approach to identify neoepitopes derived from intron retention events in tumor transcriptomes. Using mass spectrometry immunopeptidome analysis, we show that retained intron (RI) neoepitopes are processed and presented on MHC-I on the surface of cancer cell lines. RNA-derived neoepitopes should be considered for prospective personalized cancer vaccine development. 2018-08-16 2018-12 /pmc/articles/PMC6226333/ /pubmed/30114007 http://dx.doi.org/10.1038/nbt.4239 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Smart, Alicia C.
Margolis, Claire A.
Pimentel, Harold
He, Meng Xiao
Miao, Diana
Adeegbe, Dennis
Fugmann, Tim
Wong, Kwok-Kin
Van Allen, Eliezer M.
Intron retention is a source of neoepitopes in cancer
title Intron retention is a source of neoepitopes in cancer
title_full Intron retention is a source of neoepitopes in cancer
title_fullStr Intron retention is a source of neoepitopes in cancer
title_full_unstemmed Intron retention is a source of neoepitopes in cancer
title_short Intron retention is a source of neoepitopes in cancer
title_sort intron retention is a source of neoepitopes in cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226333/
https://www.ncbi.nlm.nih.gov/pubmed/30114007
http://dx.doi.org/10.1038/nbt.4239
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