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Adapting the chemical unfolding assay for high-throughput protein screening using experimental and spectroscopic corrections
The chemical unfolding (denaturation) assay can be used to calculate the change in the Gibbs free energy of unfolding, ΔG, and inflection point of unfolding, to collectively inform on molecule stability. Here, we evaluated methods for calculating the ΔG across 23 monoclonal antibody sequence variant...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226613/ https://www.ncbi.nlm.nih.gov/pubmed/30236889 http://dx.doi.org/10.1016/j.ab.2018.08.027 |