Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

In this article, we describe the method that allows fluorescently tagged structures such as axons to be targeted for electron microscopy (EM) analysis without the need to convert their labels into electron dense stains, introduce any fiducial marks, or image large volumes at high resolution. We opti...

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Detalles Bibliográficos
Autores principales: Maclachlan, Catherine, Sahlender, Daniela A., Hayashi, Shuichi, Molnár, Zoltán, Knott, Graham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232369/
https://www.ncbi.nlm.nih.gov/pubmed/30459565
http://dx.doi.org/10.3389/fnana.2018.00088

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232369/
https://www.ncbi.nlm.nih.gov/pubmed/30459565
http://dx.doi.org/10.3389/fnana.2018.00088

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