The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin

The in situ three-dimensional organization of chromatin at the nucleosome and oligonucleosome levels is unknown. Here we use cryo-electron tomography to determine the in situ structures of HeLa nucleosomes, which have canonical core structures and asymmetric, flexible linker DNA. Subtomogram remappi...

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Detalles Bibliográficos
Autores principales: Cai, Shujun, Böck, Désirée, Pilhofer, Martin, Gan, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233054/
https://www.ncbi.nlm.nih.gov/pubmed/30091658
http://dx.doi.org/10.1091/mbc.E18-05-0331
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author Cai, Shujun
Böck, Désirée
Pilhofer, Martin
Gan, Lu
author_facet Cai, Shujun
Böck, Désirée
Pilhofer, Martin
Gan, Lu
author_sort Cai, Shujun
collection PubMed
description The in situ three-dimensional organization of chromatin at the nucleosome and oligonucleosome levels is unknown. Here we use cryo-electron tomography to determine the in situ structures of HeLa nucleosomes, which have canonical core structures and asymmetric, flexible linker DNA. Subtomogram remapping suggests that sequential nucleosomes in heterochromatin follow irregular paths at the oligonucleosome level. This basic principle of higher-order repressive chromatin folding is compatible with the conformational variability of the two linker DNAs at the single-nucleosome level.
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spelling pubmed-62330542018-12-16 The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin Cai, Shujun Böck, Désirée Pilhofer, Martin Gan, Lu Mol Biol Cell Articles The in situ three-dimensional organization of chromatin at the nucleosome and oligonucleosome levels is unknown. Here we use cryo-electron tomography to determine the in situ structures of HeLa nucleosomes, which have canonical core structures and asymmetric, flexible linker DNA. Subtomogram remapping suggests that sequential nucleosomes in heterochromatin follow irregular paths at the oligonucleosome level. This basic principle of higher-order repressive chromatin folding is compatible with the conformational variability of the two linker DNAs at the single-nucleosome level. The American Society for Cell Biology 2018-10-01 /pmc/articles/PMC6233054/ /pubmed/30091658 http://dx.doi.org/10.1091/mbc.E18-05-0331 Text en © 2018 Cai, Böck, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Cai, Shujun
Böck, Désirée
Pilhofer, Martin
Gan, Lu
The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
title The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
title_full The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
title_fullStr The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
title_full_unstemmed The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
title_short The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
title_sort in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233054/
https://www.ncbi.nlm.nih.gov/pubmed/30091658
http://dx.doi.org/10.1091/mbc.E18-05-0331
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