CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses

A key goal for cell biological analyses is to assess the phenotypes that result from eliminating a target gene. Since the early 1990s, the predominant strategy utilized in human tissue culture cells has been RNA interference (RNAi)-mediated protein depletion. However, RNAi suffers well-documented of...

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Detalles Bibliográficos
Autores principales: Su, Kuan-Chung, Tsang, Mary-Jane, Emans, Neil, Cheeseman, Iain M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233062/
https://www.ncbi.nlm.nih.gov/pubmed/30091644
http://dx.doi.org/10.1091/mbc.E18-04-0214

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233062/
https://www.ncbi.nlm.nih.gov/pubmed/30091644
http://dx.doi.org/10.1091/mbc.E18-04-0214

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