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High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

Spatial structure and patterning play an important role in bacterial biofilms. Here we demonstrate an accessible method for culturing E. coli biofilms into arbitrary spatial patterns at high spatial resolution. The technique uses a genetically encoded optogenetic construct—pDawn-Ag43—that couples bi...

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Detalles Bibliográficos
Autores principales: Jin, Xiaofan, Riedel-Kruse, Ingmar H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235589/
https://www.ncbi.nlm.nih.gov/pubmed/30417874
http://dx.doi.org/10.3791/58625