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De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor

Flavonols are a flavonoid subfamily widely distributed in plants, including several ones of great importance in human and animal diet (apple, tomato, broccoli, onion, beans, tea). These polyphenolic nutraceuticals exert potent antimicrobial (membrane potential disruptors), antioxidant (free-radical...

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Autores principales: Marín, Laura, Gutiérrez-del-Río, Ignacio, Entrialgo-Cadierno, Rodrigo, Villar, Claudio J., Lombó, Felipe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237366/
https://www.ncbi.nlm.nih.gov/pubmed/30440014
http://dx.doi.org/10.1371/journal.pone.0207278
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author Marín, Laura
Gutiérrez-del-Río, Ignacio
Entrialgo-Cadierno, Rodrigo
Villar, Claudio J.
Lombó, Felipe
author_facet Marín, Laura
Gutiérrez-del-Río, Ignacio
Entrialgo-Cadierno, Rodrigo
Villar, Claudio J.
Lombó, Felipe
author_sort Marín, Laura
collection PubMed
description Flavonols are a flavonoid subfamily widely distributed in plants, including several ones of great importance in human and animal diet (apple, tomato, broccoli, onion, beans, tea). These polyphenolic nutraceuticals exert potent antimicrobial (membrane potential disruptors), antioxidant (free-radical scavengers), pharmacokinetic (CYP450 modulators), anti-inflammatory (lipoxygenase inhibitors), antiangiogenic (VEGF inhibitors) and antitumor (cyclin inhibitors) activities. Biotechnological production of these nutraceuticals, for example via heterologous biosynthesis in industrial actinomycetes, is favored since in plants these polyphenols appear as inactive glycosylated derivatives, in low concentrations or as part of complex mixtures with other polyphenolic compounds. In this work, we describe the de novo biosynthesis of three important flavonols, myricetin, kaempferol and quercetin, in the industrially relevant actinomycetes Streptomyces coelicolor and S. albus. De novo biosynthesis of kaempferol, myricetin and quercetin in actinomycetes has not been described before.
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spelling pubmed-62373662018-12-01 De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor Marín, Laura Gutiérrez-del-Río, Ignacio Entrialgo-Cadierno, Rodrigo Villar, Claudio J. Lombó, Felipe PLoS One Research Article Flavonols are a flavonoid subfamily widely distributed in plants, including several ones of great importance in human and animal diet (apple, tomato, broccoli, onion, beans, tea). These polyphenolic nutraceuticals exert potent antimicrobial (membrane potential disruptors), antioxidant (free-radical scavengers), pharmacokinetic (CYP450 modulators), anti-inflammatory (lipoxygenase inhibitors), antiangiogenic (VEGF inhibitors) and antitumor (cyclin inhibitors) activities. Biotechnological production of these nutraceuticals, for example via heterologous biosynthesis in industrial actinomycetes, is favored since in plants these polyphenols appear as inactive glycosylated derivatives, in low concentrations or as part of complex mixtures with other polyphenolic compounds. In this work, we describe the de novo biosynthesis of three important flavonols, myricetin, kaempferol and quercetin, in the industrially relevant actinomycetes Streptomyces coelicolor and S. albus. De novo biosynthesis of kaempferol, myricetin and quercetin in actinomycetes has not been described before. Public Library of Science 2018-11-15 /pmc/articles/PMC6237366/ /pubmed/30440014 http://dx.doi.org/10.1371/journal.pone.0207278 Text en © 2018 Marín et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Marín, Laura
Gutiérrez-del-Río, Ignacio
Entrialgo-Cadierno, Rodrigo
Villar, Claudio J.
Lombó, Felipe
De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
title De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
title_full De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
title_fullStr De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
title_full_unstemmed De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
title_short De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
title_sort de novo biosynthesis of myricetin, kaempferol and quercetin in streptomyces albus and streptomyces coelicolor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237366/
https://www.ncbi.nlm.nih.gov/pubmed/30440014
http://dx.doi.org/10.1371/journal.pone.0207278
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