Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vec...

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Autores principales: Ji, Xiang, Si, Xiaomin, Zhang, Yi, Zhang, Huawei, Zhang, Feng, Gao, Caixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238286/
https://www.ncbi.nlm.nih.gov/pubmed/30442181
http://dx.doi.org/10.1186/s13059-018-1580-4
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author Ji, Xiang
Si, Xiaomin
Zhang, Yi
Zhang, Huawei
Zhang, Feng
Gao, Caixia
author_facet Ji, Xiang
Si, Xiaomin
Zhang, Yi
Zhang, Huawei
Zhang, Feng
Gao, Caixia
author_sort Ji, Xiang
collection PubMed
description The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-018-1580-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-62382862018-11-23 Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system Ji, Xiang Si, Xiaomin Zhang, Yi Zhang, Huawei Zhang, Feng Gao, Caixia Genome Biol Short Report The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-018-1580-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-15 /pmc/articles/PMC6238286/ /pubmed/30442181 http://dx.doi.org/10.1186/s13059-018-1580-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Ji, Xiang
Si, Xiaomin
Zhang, Yi
Zhang, Huawei
Zhang, Feng
Gao, Caixia
Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
title Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
title_full Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
title_fullStr Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
title_full_unstemmed Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
title_short Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
title_sort conferring dna virus resistance with high specificity in plants using virus-inducible genome-editing system
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238286/
https://www.ncbi.nlm.nih.gov/pubmed/30442181
http://dx.doi.org/10.1186/s13059-018-1580-4
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