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Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus

The nematode, Haemonchus contortus, is responsible for major economic losses in the livestock industry. The management of parasites such as H. contortus has been through the use of synthetic parasiticides. This has resulted in the presence of residues in meat and milk, which affects food safety. The...

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Autores principales: Fouche, Gerda, Sakong, Bellonah M., Adenubi, Olubukola T., Pauw, Elizabeth, Leboho, Tlabo, Wellington, Kevin W., Elof, Jacobus N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AOSIS 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238697/
https://www.ncbi.nlm.nih.gov/pubmed/27543148
http://dx.doi.org/10.4102/ojvr.v83i1.1164
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author Fouche, Gerda
Sakong, Bellonah M.
Adenubi, Olubukola T.
Pauw, Elizabeth
Leboho, Tlabo
Wellington, Kevin W.
Elof, Jacobus N.
author_facet Fouche, Gerda
Sakong, Bellonah M.
Adenubi, Olubukola T.
Pauw, Elizabeth
Leboho, Tlabo
Wellington, Kevin W.
Elof, Jacobus N.
author_sort Fouche, Gerda
collection PubMed
description The nematode, Haemonchus contortus, is responsible for major economic losses in the livestock industry. The management of parasites such as H. contortus has been through the use of synthetic parasiticides. This has resulted in the presence of residues in meat and milk, which affects food safety. The development of resistance to available anthelmintics coupled with their high cost has further complicated matters. This has led to the investigation of alternative methods to manage nematodes, including the use of plants and plant extracts as a potential source of novel anthelmintics. Acetone extracts were prepared from 15 South African plant species and their anthelmintic activity determined using the egg hatch assay (EHA). The leaf extract of Cleome gynandra had the best inhibitory activity (68% ± 3%) at a concentration of 2.5 mg/mL, followed by the stem extract of Maerua angolensis (65% ± 5%). The extracts had a relatively low toxicity on Vero cells determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cellular assay.
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spelling pubmed-62386972018-11-26 Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus Fouche, Gerda Sakong, Bellonah M. Adenubi, Olubukola T. Pauw, Elizabeth Leboho, Tlabo Wellington, Kevin W. Elof, Jacobus N. Onderstepoort J Vet Res Original Research The nematode, Haemonchus contortus, is responsible for major economic losses in the livestock industry. The management of parasites such as H. contortus has been through the use of synthetic parasiticides. This has resulted in the presence of residues in meat and milk, which affects food safety. The development of resistance to available anthelmintics coupled with their high cost has further complicated matters. This has led to the investigation of alternative methods to manage nematodes, including the use of plants and plant extracts as a potential source of novel anthelmintics. Acetone extracts were prepared from 15 South African plant species and their anthelmintic activity determined using the egg hatch assay (EHA). The leaf extract of Cleome gynandra had the best inhibitory activity (68% ± 3%) at a concentration of 2.5 mg/mL, followed by the stem extract of Maerua angolensis (65% ± 5%). The extracts had a relatively low toxicity on Vero cells determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cellular assay. AOSIS 2016-07-28 /pmc/articles/PMC6238697/ /pubmed/27543148 http://dx.doi.org/10.4102/ojvr.v83i1.1164 Text en © 2016. The Authors http://creativecommons.org/licenses/by/2.0/ Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.
spellingShingle Original Research
Fouche, Gerda
Sakong, Bellonah M.
Adenubi, Olubukola T.
Pauw, Elizabeth
Leboho, Tlabo
Wellington, Kevin W.
Elof, Jacobus N.
Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus
title Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus
title_full Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus
title_fullStr Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus
title_full_unstemmed Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus
title_short Anthelmintic activity of acetone extracts from South African plants used on egg hatching of Haemonchus contortus
title_sort anthelmintic activity of acetone extracts from south african plants used on egg hatching of haemonchus contortus
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238697/
https://www.ncbi.nlm.nih.gov/pubmed/27543148
http://dx.doi.org/10.4102/ojvr.v83i1.1164
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