Enhancement of a protocol purifying T1 lipase through molecular approach

T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield...

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Detalles Bibliográficos
Autores principales: Che Hussian, Che Haznie Ayu, Raja Abd. Rahman, Raja Noor Zaliha, Thean Chor, Adam Leow, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241395/
https://www.ncbi.nlm.nih.gov/pubmed/30479887
http://dx.doi.org/10.7717/peerj.5833

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241395/
https://www.ncbi.nlm.nih.gov/pubmed/30479887
http://dx.doi.org/10.7717/peerj.5833

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