A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences

We previously developed oligoribonucleotide (ORN) interference-PCR (ORNi-PCR), in which an ORN hybridises with a complementary DNA sequence and inhibits PCR amplification across the sequence in a sequence-specific manner. Suppression of target amplification by ORNi-PCR can be used to detect nucleoti...

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Detalles Bibliográficos
Autores principales: Fujita, Toshitsugu, Yuno, Miyuki, Kitaura, Fusako, Fujii, Hodaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249316/
https://www.ncbi.nlm.nih.gov/pubmed/30464194
http://dx.doi.org/10.1038/s41598-018-35479-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249316/
https://www.ncbi.nlm.nih.gov/pubmed/30464194
http://dx.doi.org/10.1038/s41598-018-35479-0

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