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Retention Mechanism of Proteins in Hydroxyapatite Chromatography – Multimodal Interaction Based Protein Separations: A Model Study

BACKGROUND: Retention mechanism of proteins in hydroxyapatite chromatography (HAC) was investigated by linear gradient elution experiments (LGE). MATERIALS AND METHODS: Several mobile phase (buffer) solution strategies and solutes were evaluated in order to probe the relative contributions of two ad...

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Detalles Bibliográficos
Autores principales: Itoh, Daisuke, Yoshimoto, Noriko, Yamamoto, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249711/
https://www.ncbi.nlm.nih.gov/pubmed/29065831
http://dx.doi.org/10.2174/1389203718666171024122106