Cargando…

Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products

Formaldehyde is one of the most dangerous chemical compounds affecting the human health; exposure to it from food may occur naturally or by intentional addition. In this study a high performance liquid chromatography method for determination of formaldehyde in dairy products was described. The dairy...

Descripción completa

Detalles Bibliográficos
Autores principales: Sebaei, Ahmed Salem, Gomaa, Ahmed M., El-Zwahry, A. A., Emara, E. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250041/
https://www.ncbi.nlm.nih.gov/pubmed/30532782
http://dx.doi.org/10.1155/2018/2757941
_version_ 1783372880369680384
author Sebaei, Ahmed Salem
Gomaa, Ahmed M.
El-Zwahry, A. A.
Emara, E. A.
author_facet Sebaei, Ahmed Salem
Gomaa, Ahmed M.
El-Zwahry, A. A.
Emara, E. A.
author_sort Sebaei, Ahmed Salem
collection PubMed
description Formaldehyde is one of the most dangerous chemical compounds affecting the human health; exposure to it from food may occur naturally or by intentional addition. In this study a high performance liquid chromatography method for determination of formaldehyde in dairy products was described. The dairy samples were reacted and extracted with a warmed organic solvent in the presence of derivatizing agent 2,4-dinitrophenylhydrazine (DNPH) and formaldehyde; the mixture was centrifuged and followed by diode array detection. The method is validated and gives average recovery of formaldehyde at the three different levels 0.1, 5.0, and 10.0 mg/kg varied between 89% and 96%. The method is linear from the limit of quantification 0.1 mg/kg up to 10 mg/kg levels. This method is intended for formaldehyde analyses in dairy products simply with stable derivatization, minimum residue loss, excellent recovery, and accurate results with a sensitive limit of detection 0.01 mg/kg. 90 dairy samples from milk, cheese, and yogurt were investigated from seven Egyptian governorates and all samples were free from formaldehyde.
format Online
Article
Text
id pubmed-6250041
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-62500412018-12-09 Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products Sebaei, Ahmed Salem Gomaa, Ahmed M. El-Zwahry, A. A. Emara, E. A. Int J Anal Chem Research Article Formaldehyde is one of the most dangerous chemical compounds affecting the human health; exposure to it from food may occur naturally or by intentional addition. In this study a high performance liquid chromatography method for determination of formaldehyde in dairy products was described. The dairy samples were reacted and extracted with a warmed organic solvent in the presence of derivatizing agent 2,4-dinitrophenylhydrazine (DNPH) and formaldehyde; the mixture was centrifuged and followed by diode array detection. The method is validated and gives average recovery of formaldehyde at the three different levels 0.1, 5.0, and 10.0 mg/kg varied between 89% and 96%. The method is linear from the limit of quantification 0.1 mg/kg up to 10 mg/kg levels. This method is intended for formaldehyde analyses in dairy products simply with stable derivatization, minimum residue loss, excellent recovery, and accurate results with a sensitive limit of detection 0.01 mg/kg. 90 dairy samples from milk, cheese, and yogurt were investigated from seven Egyptian governorates and all samples were free from formaldehyde. Hindawi 2018-11-08 /pmc/articles/PMC6250041/ /pubmed/30532782 http://dx.doi.org/10.1155/2018/2757941 Text en Copyright © 2018 Ahmed Salem Sebaei et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sebaei, Ahmed Salem
Gomaa, Ahmed M.
El-Zwahry, A. A.
Emara, E. A.
Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products
title Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products
title_full Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products
title_fullStr Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products
title_full_unstemmed Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products
title_short Determination of Formaldehyde by HPLC with Stable Precolumn Derivatization in Egyptian Dairy Products
title_sort determination of formaldehyde by hplc with stable precolumn derivatization in egyptian dairy products
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250041/
https://www.ncbi.nlm.nih.gov/pubmed/30532782
http://dx.doi.org/10.1155/2018/2757941
work_keys_str_mv AT sebaeiahmedsalem determinationofformaldehydebyhplcwithstableprecolumnderivatizationinegyptiandairyproducts
AT gomaaahmedm determinationofformaldehydebyhplcwithstableprecolumnderivatizationinegyptiandairyproducts
AT elzwahryaa determinationofformaldehydebyhplcwithstableprecolumnderivatizationinegyptiandairyproducts
AT emaraea determinationofformaldehydebyhplcwithstableprecolumnderivatizationinegyptiandairyproducts