Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers

Flow cytometric methods for detecting and quantifying reduced intracellular thiol content using fluorescein-5-maleimide (F5M) in viable eukaryotic cells date back to 1983 (Durand and Olive [1]). There has been little development in these methodologies since that time, a period that has witnessed hug...

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Detalles Bibliográficos
Autores principales: Wadley, Alex J., Morgan, Rhys G., Heesom, Kate J., Hole, Paul S., Coles, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251017/
https://www.ncbi.nlm.nih.gov/pubmed/30505701
http://dx.doi.org/10.1016/j.mex.2018.10.013

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251017/
https://www.ncbi.nlm.nih.gov/pubmed/30505701
http://dx.doi.org/10.1016/j.mex.2018.10.013

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