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Propofol inhibited autophagy through Ca(2+)/CaMKKβ/AMPK/mTOR pathway in OGD/R-induced neuron injury

BACKGROUND: The neuroprotective role of propofol (PPF) in cerebral ischemia-reperfusion (I/R) has recently been highlighted. This study aimed to explore whether the neuroprotective mechanisms of PPF were linked to its regulation of Ca(2+)/CaMKKβ (calmodulin-dependent protein kinase kinase β)/AMPK (A...

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Detalles Bibliográficos
Autores principales: Sun, Bei, Ou, Hao, Ren, Fei, Huan, Ye, Zhong, Tao, Gao, Min, Cai, Hongwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251140/
https://www.ncbi.nlm.nih.gov/pubmed/30470173
http://dx.doi.org/10.1186/s10020-018-0054-1
Descripción
Sumario:BACKGROUND: The neuroprotective role of propofol (PPF) in cerebral ischemia-reperfusion (I/R) has recently been highlighted. This study aimed to explore whether the neuroprotective mechanisms of PPF were linked to its regulation of Ca(2+)/CaMKKβ (calmodulin-dependent protein kinase kinase β)/AMPK (AMP-activated protein kinase)/mTOR (mammalian target of rapamycin)/autophagy pathway. METHODS: Cultured primary rat cerebral cortical neurons were treated with oxygen-glucose deprivation and re-oxygenation (OGD/R) to mimic cerebral I/R injury in vitro. RESULTS: Compared with the control neurons, OGD/R exposure successfully induced neuronal I/R injury. Furthermore, OGD/R exposure notably caused autophagy induction, reflected by augmented LC3-II/LC3-I ratio and Beclin 1 expression, decreased p62 expression, and increased LC3 puncta formation. Moreover, OGD/R exposure induced elevation of intracellular Ca(2+) concentration ([Ca(2+)]i). However, PPF treatment significantly antagonized OGD/R-triggered cell injury, autophagy induction, and [Ca(2+)]i elevation. Further investigation revealed that both autophagy induction by rapamycin and [Ca(2+)]i elevation by the Ca(2+) ionophore ionomycin significantly reversed the PPF-mediated amelioration of OGD/R-triggered cell injury. Importantly, ionomycin also significantly abrogated the PPF-mediated suppression of autophagy and CaMKKβ/AMPK/mTOR signaling in OGD/R-exposed neurons. Additionally, activation of CaMKKβ/AMPK/mTOR signaling abrogated the PPF-mediated autophagy suppression. CONCLUSION: Our findings demonstrate that PPF antagonized OGD/R-triggered neuronal injury, which might be mediated, at least in part, via inhibition of autophagy through Ca(2+)/CaMKKβ/AMPK/mTOR pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s10020-018-0054-1) contains supplementary material, which is available to authorized users.