Dialogue between Staphylococcus aureus SA15 and Lactococcus garvieae strains experiencing oxidative stress

BACKGROUND: Staphylococcus aureus is an important foodborne pathogen. Lactococcus garvieae is a lactic acid bacterium found in dairy products; some of its strains are able to inhibit S. aureus growth by producing H(2)O(2). Three strains of L. garvieae from different origins were tested for their abi...

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Detalles Bibliográficos
Autores principales: Queiroux, Clothilde, Bonnet, Muriel, Saraoui, Taous, Delpech, Pierre, Veisseire, Philippe, Rifa, Etienne, Moussard, Cécile, Gagne, Geneviève, Delbès, Céline, Bornes, Stéphanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251228/
https://www.ncbi.nlm.nih.gov/pubmed/30466395
http://dx.doi.org/10.1186/s12866-018-1340-3
Descripción
Sumario:BACKGROUND: Staphylococcus aureus is an important foodborne pathogen. Lactococcus garvieae is a lactic acid bacterium found in dairy products; some of its strains are able to inhibit S. aureus growth by producing H(2)O(2). Three strains of L. garvieae from different origins were tested for their ability to inhibit S. aureus SA15 growth. Two conditions were tested, one in which H(2)O(2) was produced (high aeration) and another one in which it was not detected (low aeration). Several S. aureus genes related to stress, H(2)O(2)-response and virulence were examined in order to compare their level of expression depending on the inoculated L. garvieae strain. Simultaneous L. garvieae H(2)O(2) metabolism gene expression was followed. RESULTS: The results showed that under high aeration condition, L. garvieae strains producing H(2)O(2) (N201 and CL-1183) inhibited S. aureus SA15 growth and impaired its ability to deal with hydrogen peroxide by repressing H(2)O(2)-degrading genes. L. garvieae strains induced overexpression of S. aureus stress-response genes while cell division genes and virulence genes were repressed. A catalase treatment partially or completely restored the SA15 growth. In addition, the H(2)O(2) non-producing L. garvieae strain (Lg2) did not cause any growth inhibition. The SA15 stress-response genes were down-regulated and cell division genes expression was not affected. Under low aeration condition, while none of the strains tested exhibited H(2)O(2)-production, the 3 L. garvieae strains inhibited S. aureus SA15 growth, but to a lesser extent than under high aeration condition. CONCLUSION: Taken together, these results suggest a L. garvieae strain-specific anti-staphylococcal mechanism and an H(2)O(2) involvement in at least two of the tested L. garvieae strains. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-018-1340-3) contains supplementary material, which is available to authorized users.

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