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Selection and Characterization of DNA Aptamers for Egg White Lysozyme

We have selected aptamers binding to lysozyme from a DNA library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment. During the selection process the dissociation constant of the ssDNA pool decreased from the micromolar to the low nanomolar range within five ro...

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Detalles Bibliográficos
Autores principales: Tran, Dinh T., Janssen, Kris P. F., Pollet, Jeroen, Lammertyn, Elke, Anné, Jozef, Van Schepdael, Ann, Lammertyn, Jeroen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257241/
https://www.ncbi.nlm.nih.gov/pubmed/20335968
http://dx.doi.org/10.3390/molecules15031127